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By N. Yokian. Muhlenberg College. 2018.

Beta blockers may potentiate the negative inotropic action of other antiarrhythmics generic 0.1 mg florinef with visa. Therapeutic uses: This is useful in tachyarrhythmias cheap 0.1mg florinef with amex, in pheochromocytoma and in thyrotoxicosis crisis. It is also useful in patients with atrial fibrillation and flutter refractory to digitalis. The main adverse effects of this drug are anorexia, nausea, abdominal pain, tremor, hallucinations, peripheral neuropathy, A. It is absolutely contraindicated in patients on beta blockers, quinidine or disopyramide. Class - V drugs: Digoxin causes shortening of the atrial refractory period with small doses (vagal action) and a prolongation with the larger doses (direct action). This action is of major importance in slowing the rapid ventricular rate in patients with atrial fibrillation Diuretics Diuretics are drugs, which increase renal excretion of salt and water: are principally used to remove excessive extracellular fluid from the body. In order to understand the action of diuretics it is important to have some knowledge of the basic processes that take place in the nephron (unit structure of kidney. Approximately 180 liters of fluid is filtered from the glomerulus into the nephron per day. There are three mechanisms involved in urine formation 64 a) glomerular filtration b) tubular reabsorption c) Tubular secretion. Classification of diuretics:- Most of the diuretics used therapeutically act by interfering with sodium reabsorption by the tubules. Thiazide diuretics act by inhibiting NaCl symport at the distal convoluted tubule. Adverse effects: epigastric distress, nausea, vomiting, weakness, fatigue, dizziness, impotence, jaundice, skin rash, hypokalemia, hyperuricemia, hyperglycaemia and visual disturbance. Loop diuretics: Loop diuretics like frusemde inhibit Na - K – 2Cl symporter in the ascending limb. Adverse effects: Hypokalemia, nausea, anorexia, vomiting epigastric distress, fatigue weakness muscle cramps, drowsiness. Therapeutic uses: acute pulmonary edema, edema of cardiac, hepatic and renal disease. Hypertension, cerebral edema, in drug overdose it can be used to produce forced diuresis to facilitate more rapid elimination of drug. Potassium sparing diuretics mechanism of action: Potassium sparing diuretics (spironolactone, triamterene, amiloride) are mild diuretics causing diuresis by increasing the excretion of sodium, calcium and bicarbonate but decrease the excretion of potassium. Hyponatraemia 65 Therapeutic uses: used with conjunction with thiazides or loop diuretics in edema due to, cardiac failure nephrotic syndrome and hepatic disease. Carbonic anhydrase inhibitors: these drugs like acetazolamide inhibit the enzyme carbonic anhydrase in renal tubular cells and lead to increased excretion of bicarbonate, sodium and potassium ions in urine. Main adverse effects of these agents are drowsiness, hypokalemia, metabolic acidosis and epigastric distress. Osmotic diuretics: these drugs like mannitol and glycerine (glycerol) are freely filtered at the glomerulus and are relatively inert pharmacologically and undergo limited reabsorption by renal tubule. These are administered to increase significantly the osmolality of plasma and tubular fluid. Drugs used in hypotensive states and shock Antihypotensive drugs or agents are used to elevate a low blood pressure and may be classified as follows: I. Vasoconstrictor drugs these include: • Peripherally acting vasoconstrictors which are further divided into sympathomimetic drugs and direct vasoconstrictors. Sympathomimetics used to elevate the blood pressure include adrenaline, noradrenaline, methoxamine, phenylephrine, mephentermine and ephedrine. Treatment of shock Shock is a clinical syndrome characterized by decreased blood supply to tissues. Common signs and symptoms include oliguria, heart failure, disorientation, mental confusion, seizures, cold extremities, and comma. They play important roles in physiologic processes and also have several pharmacological significances.

The alternative sigma factor SigH regulates major components of oxidative and heat stress response in Mycobacterium tuberculosis 0.1 mg florinef. Transcription regulation by the Mycobac- terium tuberculosis alternative sigma factor SigD and its role in virulence cheap 0.1 mg florinef overnight delivery. Mycobacterium tuberculosis SigM positively regulates Esx secreted proteins and nonribosomal peptide synthetase genes and down regulates virulence-associated surface lipid synthesis. Mapping and identification of Mycobacte- rium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection. Hy- poxic response of Mycobacterium tuberculosis studied by metabolic labeling and pro- teome analysis of cellular and extracellular proteins. Transcriptional adaptation of Mycobacterium tuberculosis within macrophages: insights into the phagosomal environment. Expression profiling of host pathogen interac- tions: how Mycobacterium tuberculosis and the macrophage adapt to one another. Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope-coded affinity tag technology. The cold-shock stress response in Mycobacterium smegmatis in- duces the expression of a histone-like protein. The largest open reading frame (pks12) in the Mycobacterium tuberculosis genome is involved in patho- genesis and dimycocerosyl phthiocerol synthesis. Multiple paralogous genes related to the Streptomyces coelicolor developmental regulatory gene whiB are present in Streptomy- ces and other actinomyetes. Gap, a mycobacterial specific integral membrane protein, is requiered for glycolipid transport to the cell surface. Rsh, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry. Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemi- nation. Acute infection and macrophage sub- version by Mycobacterium tuberculosis require a specialized secretion system. Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions. Myco- bacterium tuberculosis WhiB3 interacts with ProV to affect host survival but is dispensa- ble for in vivo growth. Lipoproteins of Mycobacterium tuberculosis: an abundant and functionally diverse class of cell envelope components. A new approach for the analysis of bacterial microarray-based Comparative Genomic Hybridization: insights from an empirical study. Differential expression of iron-, carbon-, and oxy- gen-responsive mycobacterial genes in the lungs of chronically infected mice and tuber- culosis patients. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis. Effect of slow growth on metabolism of Escherichia coli, as revealed by global metabolite pool ("metabolome") analysis. Comparison of predicted and observed properties of proteins encoded in the genome of Mycobacterium tuberculosis H37Rv. Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. Integrating metabolomics into a systems biology framework to exploit metabolic complexity: strategies and applications in micro- organisms. An in vitro model for sequential study of shiftdown of Mycobacte- rium tuberculosis through two stages of nonreplicating persistence. Recently, it has become clear that, in order to develop a more efficient vaccine, a better understanding of the relation between the immune re- sponse of the host and the tubercle bacillus is needed. In view of this, the present chapter provides an updated overview of the cellular and molecular immune mechanisms involved in the development of the disease. Neutrophil leukocytes Even though macrophages are considered the main targets for infection by Myco- bacterium tuberculosis, it has been recently proposed that other cell populations can also be infected by mycobacteria and therefore may be important in the devel- opment of the disease.

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The diagnosis of such diseases need experienced ophthalmic worker buy cheap florinef 0.1mg on line, appropriate instruments and especial diagnostic tests and procedures purchase 0.1 mg florinef amex. Their visual out come highly depends on the time interval between onset of the disease and initiation of treatment and subsequent close follow up. Symptoms - Painful red eye - Sudden reduction of vision - Rapid progressive visual impairment. Symptoms - Painful disorder-typically a constant severe boring pain that worsens at night or in the early morning hours and radiates to the face and 42 periorbital region. To give a general over view on the burden of blindness on global and country levels 2. To give a clear idea on the disease that have been launched by vision 2020 to control disease 4. The hope is that by the year 2020 most of the avoidable blindness in the world should be eliminated, so that everyone in the world except those with untreatable and unavoidable disease should have a visual acuity of 20/20 by the year 2020. The three main components (priorities) of Vision 2020 are 1-human resources development 2- Infrastructure and appropriate technology 3- Disease control (cataract, trachoma, onchocercaisis, childhood blindness, refractive error glaucoma and low vision). The result of these two factors means that the population aged over 60 years will double during the next 20 yrs from approximately 400 million now, to around 800 million in 2020. This increase in the elderly population will result in a greater number of the people with visual loss and blindness from cataract that will need eye services. A figure of 1000 new blind people from cataract per million populations per year is used for planning purpose in developing countries. Progress of the disease Some patient develops mature cataract only in a few months after a sign of opacity in the lens, others with early opacity may persist in the lens for many years without obvious progress at all. Signs - Reduced V/A - Whitish opacity seen through the pupil(see color plate3) Complication of unoperated cataract ™ Dislocation or sublaxation of lens ™ Glaucoma ™ Uveitis Operable cataract eyes The term is used to define a cataract where the patient and the surgeon agree to proceed with cataract surgery. It is a Greek word meaning ‘rough’ which describes the surface appearance of the conjunctiva. Trachoma tends to be found in dry rural areas, where lack of water and bad living conditions may facilitate the spread of the disease. Trachmatos inflammation becomes increasingly intense in children up to the age of six to eight years. Scars on the inside of the eye lids, caused by trachoma, can be found in children from the age of four years. Scarring is increasingly common in older children, but the serious complication of inturned eye lashes and corneal scarring do not usually appear before adult age. Trachoma in the community The severity of trachoma can vary from one community to another because of differences in the eases of spread of infection. Children are the main reservoir of Trachomatous infection, as they are commonly and heavily infected. Compared to men, women tend to have more severe trachoma, including inturned eyelashes and blindness, probably re infected by children for whom they care. Central and sufficiently dense to obscure the part of pupil Aims of simplified trachoma grading 1. To facilitate all health workers the recognition of the signs of trachoma and its complications 2. To enable health workers to assist in undertaking simple surveys to identify communities in need of measures to control blindness from trachoma. To allow for easy evaluation, by health workers, of results of trachoma control efforts in identified communities. Other terms, commonly used in older classification of trachoma, can be related to the present scheme. The number of people with Trichiasis (1%or more); this indicates the immediate need to provide surgical services for lid correction 5. Doxycycline 100mg po/d for 21 days, don’t give for children below 7 years, pregnant and lactating mother B. For community treatment of trachoma, mass distribution of tetracycline ointment is carried out especially during epidemics of conjunctivitis at intervals for five consecutive days per month for six months. The eye acts as a convex lens and parallel rays of light are focused on the retina. For this reason during distance vision testing the patient is seated 6 meters from the test chart. Most of the refraction in the eye is done by the cornea (2/3) the rest being by the lens (1/3) Fig 5.

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